Journal: Journal of Ginseng Research

Article Title: Enhancing effect of Panax ginseng on Zip4-mediated zinc influx into the cytosol

doi: 10.1016/j.jgr.2021.06.006

Figure Lengend Snippet: Effects of ginsenosides on luciferase activity driven by intracellular zinc levels. Hepa cells, co-transfected with the pGL4.26-MRE and the pGL4.74 plasmids, were incubated in normal medium (control) and the indicated concentrations of ginsenoside Rb1 (A), Rb2 (B), Rc (C), Rd (D), Re (E), Rf (F), Rg1 (G), and Rg2 (H) for 24 h. ∗ P < 0.05, significantly different from control. Each bar represents the mean ± S.D. (n = 3).

Article Snippet: Ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2 were obtained from Biosynth Carbosynth (Berkshire, United Kingdom).

Techniques: Luciferase, Activity Assay, Transfection, Incubation, Control

Journal: Journal of Ginseng Research

Article Title: Enhancing effect of Panax ginseng on Zip4-mediated zinc influx into the cytosol

doi: 10.1016/j.jgr.2021.06.006

Figure Lengend Snippet: Content of Ginsenosides in 1000 μg/mL of P. ginseng Extract

Article Snippet: Ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2 were obtained from Biosynth Carbosynth (Berkshire, United Kingdom).

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

doi: 10.1155/2020/4764219

Figure Lengend Snippet: Compounds identified from white ginseng and extruded white ginseng.

Article Snippet: Ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rg2, ginsenoside Ro, and standard chemicals were purchased from Shanghai Standard Technology Co., Ltd. (purity ≥ 98%, Shanghai, China).

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Chemical Comparison of White Ginseng before and after Extrusion by UHPLC-Q-Orbitrap-MS/MS and Multivariate Statistical Analysis

doi: 10.1155/2020/4764219

Figure Lengend Snippet: The heat maps visualizing the intensities of the 17 ginsenoside datasets from white ginseng and extruded white ginseng ( n = 10) and extruded white ginseng ( n = 10).

Article Snippet: Ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rg2, ginsenoside Ro, and standard chemicals were purchased from Shanghai Standard Technology Co., Ltd. (purity ≥ 98%, Shanghai, China).

Techniques:

Journal: Genesis (New York, N.Y. : 2000)

Article Title: FUCCI tracking shows cell-cycle-dependent Neurog3 variation in pancreatic progenitors

doi: 10.1002/dvg.23050

Figure Lengend Snippet: Generation of an LCA-capable BAC transgenic Neurog3RG mESC line. (A) Schematic detailing the generation of transgenic mES cell lines carrying a single copy of a Neurog3RG BAC transgene designed to serve as an LCA in future RMCE reactions. The Neurog3RG BAC transgenic mESCs were previously used to generate Neurog3RG reporter mice (Bechard et al., 2016). Neurog3 5′ untranslated region (UTR) represents the region 5′ of the start codon containing cis regulatory elements and the Neurog3 5′/UTR. (B) Table and graph of a standard curve, generated via a qPCR-based assay (see methods and materials), that relates transgene copy number to a specific CT value. (C) Table and graph depicting the estimated Neurog3RG BAC transgene copy number present in Neurog3RG1 and Neurog3RG2 mESC lines. Each data point represents the average of the indicated numbers of qPCR runs ± SEM.

Article Snippet: Generation of Neurog3 RG2 and Neurog3 P2A.FUCCI mice was supported in part by the Beta Cell Biology Consortium Mouse ES Cell Core funded by the NIDDK (U01DK072473).

Techniques: Transgenic Assay, Generated

Journal: Genesis (New York, N.Y. : 2000)

Article Title: FUCCI tracking shows cell-cycle-dependent Neurog3 variation in pancreatic progenitors

doi: 10.1002/dvg.23050

Figure Lengend Snippet: Neurog3 protein levels vary according to cell-cycle phase. (A) E14.5 pancreatic epithelium showing Sox9, Neurog3, hCdt1mKO2 and hGemmVenus. Red arrowheads indicate Neurog3P2A.FUCCI+ cells that are mKO2+ and thus in G1, green arrowheads indicate Neurog3P2A.FUCCI+ cells that are mVenus+ and thus in S-G2-M phase. Asterisk indicates Sox9+ Neurog3TA.LO cells, arrows with no asterisk indicate Sox9− Neurog3TA.HI cells. (B) Left, percentage of Sox9+ Neurog3TA.LO and Sox9− Neurog3TA.HI cells in S-G2-M versus G1 phase. Right, percentage of Sox9+ Neurog3TA.pLO and Sox9+ Neurog3TA.pUD in S-G2-M versus G1 phase. (n = 1600, N = 3). (*) P = 0.0072; (**) P = 4 x 10−6; (***) P = 0.0607; (****) P = 0.0039. Each data point is an average of N = 3 with error bars representing ± SEM. Scale bars, 20 μm.

Article Snippet: Generation of Neurog3 RG2 and Neurog3 P2A.FUCCI mice was supported in part by the Beta Cell Biology Consortium Mouse ES Cell Core funded by the NIDDK (U01DK072473).

Techniques:

Journal: Genesis (New York, N.Y. : 2000)

Article Title: FUCCI tracking shows cell-cycle-dependent Neurog3 variation in pancreatic progenitors

doi: 10.1002/dvg.23050

Figure Lengend Snippet: Neurog3 promotes low-level activation of downstream targets during G1 in the mitotic Neurog3TA.LO progenitor state. (A) flow cytometry plot detailing capture of lumen-apposed (Muc1+) Neurog3P2A.FUCCI+ cells in S-G2-M (mKO2− mVenus+) (Blue population) or G1 (mKO2+ mVenus−) (red population) from E14.5 Neurog3P2A.FUCCI pancreata. Flow-sorted cells were collected into TRIzol for RNA isolation and cDNA synthesis. (B) Relative expression level (y-axis), normalized to Gapdh, of Sox9, Neurog3, Hes1, NeuroD1, Insm1, glucagon, and Insulin for E14.5 flow captured Muc1+ Neurog3P2A.FUCCI+ mKO2− mVenus+ (blue bars) and Muc1+ Neurog3P2A.FUCCI mKO2+ mVenus− (red bars) cells. Unless where noted differently on the figure, data points are an average of n = 6 technical replicates with error bars representing ±SEM. See supplemental table 1 for a list of primers used.

Article Snippet: Generation of Neurog3 RG2 and Neurog3 P2A.FUCCI mice was supported in part by the Beta Cell Biology Consortium Mouse ES Cell Core funded by the NIDDK (U01DK072473).

Techniques: Activation Assay, Flow Cytometry, Isolation, cDNA Synthesis, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

doi: 10.3390/ijms18061114

Figure Lengend Snippet: In-house library for the analysis of 30 ginsenosides using UPLC-QTOF/MS.

Article Snippet: Ginsenoside Rg1, Re, Rg2, Rb1, Rc, Rb2, Rb3, and Rd standards were purchased from Chromadex (Irvine, CA, USA), and ginsenoside 20(S)-Rg2 and CK were obtained from the Ambo Institute (Seoul, Korea).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

doi: 10.3390/ijms18061114

Figure Lengend Snippet: Validation study for the analysis of 30 ginsenosides using UPLC-QTOF/MS.

Article Snippet: Ginsenoside Rg1, Re, Rg2, Rb1, Rc, Rb2, Rb3, and Rd standards were purchased from Chromadex (Irvine, CA, USA), and ginsenoside 20(S)-Rg2 and CK were obtained from the Ambo Institute (Seoul, Korea).

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

doi: 10.3390/ijms18061114

Figure Lengend Snippet: Two merged MALDI-TOF/MS spectra of seven ginsenosides (Rh1, Rg2, Rg4, Rg1, Re, Rb2, and Rb1) obtained using the 2,5-dihydroxybenzoic acid (DHB) ( blue ) and α-cyano-4-hydroxy-cinnamic acid (CHCA) ( red ) matrices, respectively. In positive ion mode, seven ginsenosides were detected with the adduction of sodium (Na) or potassium (K).

Article Snippet: Ginsenoside Rg1, Re, Rg2, Rb1, Rc, Rb2, Rb3, and Rd standards were purchased from Chromadex (Irvine, CA, USA), and ginsenoside 20(S)-Rg2 and CK were obtained from the Ambo Institute (Seoul, Korea).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mass Spectrometry Based Profiling and Imaging of Various Ginsenosides from Panax ginseng Roots at Different Ages

doi: 10.3390/ijms18061114

Figure Lengend Snippet: MALDI-MSI cross sections showing the localization of ( A ) Rh1 ([M + K] + , m / z 677.9), ( B ) Rg2 ([M + K] + , m / z 823.3), ( C ) Rc or Rb2 or Rb3 ([M + K] + , m / z 1117.5), and ( D ) Rb1 ([M + K] + , m / z 1147.6) from ginseng roots cultivated for 4, 5, and 6 years.

Article Snippet: Ginsenoside Rg1, Re, Rg2, Rb1, Rc, Rb2, Rb3, and Rd standards were purchased from Chromadex (Irvine, CA, USA), and ginsenoside 20(S)-Rg2 and CK were obtained from the Ambo Institute (Seoul, Korea).

Techniques: